Effect of caspases and RANKL induced by heavy force in orthodontic root resorption
Minato Yukari, Yamaguchi Masaru, Shimizu Mami, Kikuta Jun, Hikida Takuji, Hikida Momoko, Suemitsu Masaaki, Kuyama Kayo, Kasai Kazutaka,
소속 상세정보
( Minato Yukari ) - Japan Nihon University School of Dentistry at Matsudo Department of Orthodontics
( Yamaguchi Masaru ) - Japan Nihon University School of Dentistry at Matsudo Department of Orthodontics
( Shimizu Mami ) - Japan Nihon University School of Dentistry at Matsudo Department of Orthodontics
( Kikuta Jun ) - Japan Nihon University School of Dentistry at Matsudo Department of Orthodontics
( Hikida Takuji ) - Japan Nihon University School of Dentistry at Matsudo Department of Orthodontics
( Hikida Momoko ) - Japan Nihon University School of Dentistry at Matsudo Department of Orthodontics
( Suemitsu Masaaki ) - Nihon University School of Dentistry at Matsudo Department of Oral Pathology
( Kuyama Kayo ) - Nihon University School of Dentistry at Matsudo Department of Oral Pathology
( Kasai Kazutaka ) - Japan Nihon University School of Dentistry at Matsudo Department of Orthodontics
Abstract
Objective: Orthodontic root resorption (ORR) due to orthodontic tooth movement is a difficult treatment-related adverse event. Caspases are important effector molecules for apoptosis. At present, little is known about the mechanisms underlying ORR and apoptosis in the cementum. The aim of the present in vivo study was to investigate the expression of tartrate-resistant acid phosphatase (TRAP), caspase 3, caspase 8, and receptor activator of nuclear factor kappa-B ligand (RANKL) in the cementum in response to a heavy or an optimum orthodontic force.
Methods: The maxillary molars of male Wistar rats were subjected to an orthodontic force of 10 g or 50 g using a closed coil spring. The rats were sacrificed each experimental period on days 1, 3, 5, and 7 after orthodontic force application. And the rats were subjected to histopathological and immunohistochemical analyses.
Results: On day 7 for the 50-g group, hematoxylin and eosin staining revealed numerous root resorption lacunae with odontoclasts on the root, while immunohistochemistry showed increased TRAP- and RANKL-positive cells. Caspase 3- and caspase 8-positive cells were increased on the cementum surfaces in the 50-g group on days 3 and 5. Moreover, the number of caspase 3- and caspase 8-positive cells and RANKLpositive cells was significantly higher in the 50-g group than in the 10-g group.
Conclusions: In our rat model, ORR occurred after apoptosis was induced in the cementum by a heavy orthodontic force. These findings suggest that apoptosis of cementoblasts is involved in ORR.
키워드
Apoptosis; Caspase; Receptor activator of nuclear factor kappa-B ligand; Root resorption
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